To investigate high yield citric acid producers other than citrus fruits, Aspergillus niger was used for submerged citric acid synthesis. For a comparative investigation of higher yielding substrates, rice and potato extracts were used as substrates. The changes in citric acid output were tracked using different amounts of Sucrose, Glucose, and Nitrogen supplementation. The concentration of citric acid generated was determined by titrating citric acid extracted from various mediums. The objective of the comparison study was to determine the ideal requirements for a greater yield of citric acid production. This research could help with large-scale industrial manufacturing of citric acid, which is one of the most widely used organic acids.
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