COMPARATIVE ANALYSIS OF CITRIC ACID PRODUCTION BY Aspergillus niger USING DIFFERENT MEDIA | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

To investigate high yield citric acid producers other than citrus fruits, Aspergillus niger was used for submerged citric acid synthesis. For a comparative investigation of higher yielding substrates, rice and potato extracts were used as substrates. The changes in citric acid output were tracked using different amounts of Sucrose, Glucose, and Nitrogen supplementation. The concentration of citric acid generated was determined by titrating citric acid extracted from various mediums. The objective of the comparison study was to determine the ideal requirements for a greater yield of citric acid production. This research could help with large-scale industrial manufacturing of citric acid, which is one of the most widely used organic acids.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5858

In-vitro ANTIFUNGAL STUDY OF CRUDE EXTRACT OF Barringtonia asiatica (L.) Kurtz AGAINST FUNGAL PATHOGENIC STRAINS | Asian Journal of Microbiology and Biotechnology,

Introduction: Barringtonia asiatica (L.) Kurtz is a plant native to tropical mangrove ecosystems and belongs to the Lecythidaceae family. The goal of this study was to see how effective a crude extract of Barringtonia asiatica (L.) Kurtz was against fungal pathogenic strains in vitro.

Materials and Methods: In order of increasing polarity, extractions were carried out using the traditional method of cold-soaking in non-polar, medium polar, and polar solvents (hexane, dichloromethane, chloroform, ethyl acetate and methanol). The antifungal activity of Candida tropicalis, Fusarium oxysporum, Aspergillus niger, and Aspergillus flavin was assessed using a disc diffusion assay with Potato dextrose agar medium (PDA), Diflucan as a control and Candida tropicalis, Fusarium oxysporum, Aspergillus niger, and Aspergillus flavin as pathogens.

Results: At 500 g/mL, hexane extract inhibited Aspergillus niger significantly, with an inhibition zone of 14.770.05 mm. With an inhibition zone of 15.93 0.05 mm, dichloromethane crude leaf extract inhibited Fusarium oxysporium growth more effectively.

Conclusion: The goal of this work is to show that Barringtonia asiatica may be used in a variety of solvents, including non-polar and polar crude extracts, to battle antifungal resistance and diseases resistant to contemporary medicine.

Please see the link :- https://www.ikprress.org/index.php/AJMAB/article/view/5091

COMPARATIVE ANALYSIS OF CITRIC ACID PRODUCTION BY Aspergillus niger USING DIFFERENT MEDIA | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

To investigate high yield citric acid producers other than citrus fruits, Aspergillus niger was used for submerged citric acid synthesis. For a comparative investigation of higher yielding substrates, rice and potato extracts were used as substrates. The changes in citric acid output were tracked using different amounts of Sucrose, Glucose, and Nitrogen supplementation. The concentration of citric acid generated was determined by titrating citric acid extracted from various mediums. The objective of the comparison study was to determine the ideal requirements for a greater yield of citric acid production. This research could help with large-scale industrial manufacturing of citric acid, which is one of the most widely used organic acids.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/5858

In-vitro ANTIFUNGAL STUDY OF CRUDE EXTRACT OF Barringtonia asiatica (L.) Kurtz AGAINST FUNGAL PATHOGENIC STRAINS | Asian Journal of Microbiology and Biotechnology

Introduction: Barringtonia asiatica (L.) Kurtz belong to a Family of Lecythidaceae is a species native to mangrove habitats in the tropical. The aim of this study was to evaluate the In-vitro Antifungal Study of Crude extract of Barringtonia asiatica (L.) Kurtz against Fungal Pathogenic Strains.

Materials and Methods: Extractions was carried out through conventional method, using cold-soaking method in non-polar, medium polar and polar solvents in the order of increasing polarity (hexane, dichloromethane, chloroform, ethyl acetate and methanol). Antifungal activity was evaluated using disc diffusion assay, using Potato dextrose agar medium (PDA), Diflucan as control and Candida tropicalis, Fusarium oxysporum, Aspergillus niger and Aspergillus flavin as pathogen were utilized.

Results: Significant inhibition of hexane extract was observed on Aspergillus niger at 500 µg/mL with inhibition zone of 14.77±0.05 mm. Dichloromethane crude leaf extract exhibited higher growth inhibition against Fusarium oxysporium with inhibition zone of 15.93 ± 0.05 mm.

Conclusion: The focus of this study is to provide the prospects of Barringtonia asiatica different solvent from non-polar and polar crude extract as a good agent for combating antifungal menace and diseases resistance from modern medicine.

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